Applications of studying Electrophoretic pattern of Haemonchus contortus as tool for vaccine designing - A Review

The study of electrophoretic pattern of Haemonchus contortus has proved to be an important tool for vaccine designing. The aim behind the review is to encourage young researchers to initiate work on this aspect. Disease biomarker discovery is generally carried out using two dimensional polyacrylamide gel Electrophoresis (2D-PAGE) to identify differences in the protein expression patterns. After 2D-PAGE fractionation and staining, the protein(s) of interest are removed, proteolytically or chemically digested and identified by mass spectrometry (MS). Although 2DPAGE separation provides excellent resolution, the need for protein staining and the subsequent sample handling limits the sensitivity of the overall approach. Protein profiling is expected to discover unexpected targets for drug design by determining the function of thousands of unidentified proteins still likely to be found in the genome of Haemonchus contortus. Protein profiling is expected to multiply the number of known drug targets 100fold. This will encourage the pharmaceutical industry to develop new drugs against this economically important parasite. This review will focus on research carried out globally by the applications of electrophoresis and so far various proteins have been identified as targets for vaccine designing. [Irfan-ur-Rauf Tak, M. Z. Chishti and Fayaz Ahmad. Applications of studying Electrophoretic pattern of Haemonchus contortus as tool for vaccine designing A Review. N Y Sci J 2013;6(9):60-65]. (ISSN: 1554-0200). http://www.sciencepub.net/newyork. 9